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Báo cáo y học: "Novel model for end-neuroma formation in the amputated rabbit forelimb"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Novel model for end-neuroma formation in the amputated rabbit forelimb. | Kim et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 6 http www.jbppni.eom content 5 1 6 JOURNAL OF BRACHIAL PLEXUS AND PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access Novel model for end-neuroma formation in the amputated rabbit forelimb Peter S Kim1 Jason Ko1 Kristina K O Shaughnessy1 Todd A Kuiken2 Gregory A Dumanian1 Abstract Background The forelimb amputee poses many reconstructive challenges in the clinical setting and there is a paucity of established surgical models for study. To further elucidate the pathogenic process in amputation neuroma formation we created a reproducible well-tolerated rabbit forelimb amputation model. Methods Upon approval from the Institutional Animal Care and Use Committee 5 New Zealand White rabbits underwent left forelimb amputation. During this initial surgery the median radial and ulnar nerves were transected 1.6-2.5 mean 2.0 cm distal to the brachial plexus transposed onto the anterior chest wall and preserved at length. Six weeks subsequent to the amputation the distal 5 mm of each neuroma was excised and the remaining stump underwent histomorphometric analysis. Results The nerve cross sectional areas increased by factors of 1.99 3.17 and 2.59 in the median p 0.077 radial p 0.0001 and the ulnar p 0.0026 nerves respectively. At the axonal level the number and cross-sectional area of myelinated fibers demonstrated an inverse relationship whereby the number of myelinated fibers in the median radial and ulnar nerves increased by factors of 5.13 p 0.0043 5.25 p 0.0056 and 5.59 p 0.0027 and the cross-sectional areas of these myelinated fibers decreased by factors of 4.62 p 0.001 3.51 p 0.01 and 4.29 p 0.0259 respectively. Conclusion Given that the surgical model appears well-tolerated by the rabbits and that patterns of morphologic change are consistent and reproducible we are encouraged to further investigate the utility of this model in the pathogenesis of neuroma formation. Introduction In the modern era