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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families, and releasing of cytochrome c | Ling et al. Journal of Experimental Clinical Cancer Research 2011 30 66 http www.jeccr.eom content 30 1 66 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Oxymatrine induces human pancreatic cancer PANC-1 cells apoptosis via regulating expression of Bcl-2 and IAP families and releasing of cytochrome c Qi Ling Xiao Xu Xuyong Wei Weibing Wang Bin Zhou Bei Wang and Shusen Zheng Abstract Background Oxymatrine an isolated extract from traditional Chinese herb Sophora Flavescens Ait has been traditionally used for therapy of anti-hepatitis B virus anti-inflammation and anti-anaphylaxis. The present study was to investigate the anti-cancer effect of oxymatrine on human pancreatic cancer PANC-1 cells and its possible molecular mechanism. Methods The effect of oxymatrine on the viability and apoptosis was examined by methyl thiazolyl tetrazolium and flow cytometry analysis. The expression of Bax Bcl-2 Bcl-x L S Bid Bad HIAP-1 HIAP-2 XIAP NAIP Livin and Survivin genes was accessed by RT-PCR. The levels of cytochrome c and caspase 3 protein were assessed by Western blotting. Results Oxymatrine inhibited cell viability and induced apoptosis of PANC-1 cells in a time- and dose-dependent manner. This was accompanied by down-regulated expression of Livin and Survivin genes while the Bax Bcl-2 ratio was upregulated. Furthermore oxymatrine treatment led to the release of cytochrome c and activation of caspase-3 proteins. Conclusion Oxymatrine can induce apoptotic cell death of human pancreatic cancer which might be attributed to the regulation of Bcl-2 and IAP families release of mitochondrial cytochrome c and activation of caspase-3. Background Pancreatic cancer is one of the most common malignant tumors worldwide. It can only be managed with surgical treatment in limited cases whereas the majority of cases presented advanced tumors responds poorly to current available medical therapies 1 . Similar to other tumor types insufficient cell death and or excessive