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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro"
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro | Jiang et al. Journal of Experimental Clinical Cancer Research 2011 30 60 http www.jeccr.eom content 30 1 60 Journal of Experimental Clinical Cancer Research RESEARCH Open Access ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha MMP-9 CyclinD1 and invasiveness proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro Qingming Jiang Hui Zhang and Ping Zhangt Abstract Background MTA1 metastasis associated-1 is a tumor metastasis associated candidate gene and overexpression in many human tumors including breast cancer. In this study we investigated depressive effect on MTA1 by MTA1-specific short hairpin RNA shRNA expression plasmids in human breast cancer cell lines MDA-MB-231 and MCF-7 and effect on protein levels of ER alpha MMP-9 cyclinD1 and tumor cell invasion proliferation. Methods ShRNA expression vectors targeting MTA1 was constructed and transfected into human breast cancer cell lines MDA-MB-231 and MCF-7. The transfection efficiency was evaluated by fluorescence microscopy mRNA levels of MTA1 were detected by reverse transcription-polymerase chain reaction RT-PCR protein levels of ER alpha MMP-9 and cyclinD1 were detected by Western blotting respectively. Tumor cells invasive ability were evaluated by Boyden chamber assay the cells proliferation were evaluated using cell growth curve and MTT analysis the cell cycle analysis was performed using flow cytometry. Results Down-regulation of MTA1 by RNAi approach led to re-expression of ER alpha in ER-negative breast cancer cell lines MDA-MB-231 and reduced protein levels of MMP-9 and CyclinD1 as well as decreased tumor cell invasion and proliferation more cells were blocked in G0 G1 stage P 0.05 . However after inhibiting mRNA levels of MTA1 protein expression of ER alpha MMP-9 cyclinD1 and the changes of cancer cells invasiveness proliferation cells cycle were no statistical difference in ER-positive human breast cancer cell lines MCF-7 P 0.05 . Conclusions ShRNA .