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Handbook of Microbiological Media, Fourth Edition part 66

Handbook of Microbiological Media, Fourth Edition part 66 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Enrichment Broth pH 7.3 with Pyruvate 645 Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Prepare freshly or boil and cool the medium just before use. Use For the cultivation of both aerobic and anaerobic organisms. For the performance of sterility tests of turbid or viscous specimens. Enrichment Broth for Aeromonas hydrophila Composition per liter NaCl.5.0g Maltose.3.5g Yeast extract.3.0g Bile salts No. 3.1.0g L-Cysteine-HCl-H2O.0.3g Bromthymol Blue.0.03g Novobiocin.5.0mg pH 7.0 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and enrichment of Aeromonas hydrophila. Enrichment Broth pH 7.3 Composition per liter Pancreatic digest of casein.17.0g Yeast extract.6.0g NaCl.5.0g Papaic digest of soybean meal.3.0g Glucose.2.5g K2HPO4.2.5g Nalidixic acid solution.8.0mL Cycloheximide solution.5.1mL Acriflavin-HCl solution.3.0mL pH 7.3 0.2 at 25 C Cycloheximide Solution Composition per 10.0mL Cycloheximide.0.1g Ethanol absolute.4.0mL Preparation of Cycloheximide Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Nalidixic Acid Solution Composition per 10.0mL Nalidixic acid. 0.05g Preparation of Nalidixic Acid Solution Add nalidixic acid to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Acriflavin-HCl Solution Composition per 10.0mL Acriflavin-HCl.0.05g Preparation of Acriflavin-HCl Solution Add acriflavin-HCl to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except cycloheximide solution nalidixic .