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Handbook of Microbiological Media, Fourth Edition part 181

Handbook of Microbiological Media, Fourth Edition part 181 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | TMA Mineral Medium 1795 Glutamine solution.6.0mL CaCl2-2H2O solution optional .2.0mL pH 7.2-7.4 at 25 C Glutamine Solution Composition per 100.0mL L-Glutamine.5.0g NaCl 0.85 solution .100.0mL Preparation of Glutamine Solution Add the glutamine to the 0.85 NaCl solution. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glutamine and serum to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2-7.4 with 10 Na2CO3 solution. Filter sterilize. Aseptically add 6.0mL of sterile glutamine solution and 50.0100.0mL of sterile serum. Human serum bovine serum horse serum or fetal calf serum may be used. Mix thoroughly. To grow cells in a monolayer aseptically add 2.0mL of a sterile 10 CaCl2-2H2O solution. To grow cells in suspension omit the CaCl2-2H2O solution. Use For preparation of Eagle s minimal medium for the cultivation of cells in monolayer or suspension in tissue culture. Tissue Culture Minimal Medium Eagle Spinner Modified TC Minimal Medium Eagle Spinner Modified MEM-S Composition per 1056.0mL NaH2PO4.1.35g NaCl.6.8g NaHCO3.2.2g Glucose.1.0g KCl.0.4g CaCl22H2O.0.2g NaH2PO4.0.125g MgSO4-7H2O.0.1g L-Isoleucine.0.026g L-Leucine.0.026g L-Lysine.0.026g L-Threonine.0.024g L-Valine.0.0235g L-Tyrosine.0.018g L-Arginine.0.0174g L-Phenylalanine.0.0165g L-Cystine.0.012g L-Histidine.8.0mg L-Methionine.7.5mg Phenol Red.5.0mg L-Tryptophan.4.0mg Inositol.1.8mg Biotin.1.0mg Calcium pantothenate.1.0mg Choline chloride.1.0mg Folic acid.1.0mg Nicotinamide.1.0mg Pyridoxal-HCl.1.0mg Thiamine-HCl.1.0mg Riboflavin.0.1mg Serum.50.0mL-100.0mL Glutamine solution.6.0mL pH 7.2-7.4 at 25 C Glutamine Solution Composition per 100.0mL L-Glutamine . 5.0g NaCl 0.85 solution .100.0mL Preparation of Glutamine Solution Add the glutamine to the 0.85 NaCl solution. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glutamine and serum to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to .