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báo cáo hóa học: " Overexpression of serine racemase in retina and overproduction of D-serine in eyes of streptozotocin-induced diabetic retinopathy"
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Overexpression of serine racemase in retina and overproduction of D-serine in eyes of streptozotocin-induced diabetic retinopathy | Jiang et al. Journal of Neuroinflammation 2011 8 119 http www.jneuroinflammation.eom content 8 1 119 JIOURNAL OF. NEUROINFLAMMATION RESEARCH Open Access Overexpression of serine racemase in retina and overproduction of D-serine in eyes of streptozotocin-induced diabetic retinopathy I_I z s I I 1 2 11 I U Z I c z t 1 2 Dr A 12 f _ I 11 I . I i U . 1 2 I I L Cl IL . 1 2 11 hl I 1 2 Cbz v ĩ U . A D n r M 4 5 s U . Haiyan Jiang Junxu rang Bo vvu Guibin Yin Lin Sun Jia Qu Steven W Barger ana Shengzhou Wu1 2 Abstract Background Recent data indicate that inflammatory mechanisms contribute to diabetic retinopathy DR . We have determined that serine racemase SR expression is increased by inflammatory stimuli including liposaccharide LPS amyloid b-peptide A-beta and secreted amyloid precursor protein sAPP expression is decreased by the anti-inflammatory drug dexamethasone. We tested possibility that SR and its product D-serine were altered in a rat model of DR. Methods Intraperitoneal injection of streptozotocin STZ 70 mg kg body weight to Sprague-Dawley rats produced type-I diabetic mellitus fasting blood sugar higher than 300 mg dL . At 3 and 5 months after STZ or saline injection retinas from some rats were subjected to cryosectioning for immunofluorescent analysis of SR and TUNEL assay of apoptosis. Retinal homogenates were used to detect SR levels and Jun N-terminal kinase JNK activation by immunoblotting. Aqueous humor and retina were also collected to assay for neurotransmitters including glutamate and D-serine by reverse-phase HPLC. Results Compared to saline-injected rats STZ-injected diabetic rats showed elevation of SR protein levels in retinal homogenates attributed to the inner nuclear layer INL by immunofluorescence. Aqueous humor fluid from STZ-injected rats contained significantly higher levels of glutamate and D-serine compared to controls by contrast D-serine levels in retinas did not differ. Levels of activated JNK were elevated in diabetic retinas .