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báo cáo hóa học: " Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9 | Journal of Neuroinflammation BioMed Central Research Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9 Timothy J Cunningham Jaquie Maciejewski and Lihua Yao Open Access Address Department of Neurobiology and Anatomy Drexel University College of Medicine 2900 Queen Lane Philadelphia PA 19129 USA Email Timothy J Cunningham - tcunning@drexelmed.edu Jaquie Maciejewski - jacquiemace@gmail.com Lihua Yao - lihuayao@yahoo.com Corresponding author Published II September 2006 Received 10 June 2006 Accepted 11 September 2006 Journal of Neuroinflammation 2006 3 25 doi 10.1186 1742-2094-3-25 This article is available from http www.jneuroinflammation.cOm content 3 1 25 2006 Cunningham et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The nonapeptide CHEC-9 CHEASAAQC a putative inhibitor of secreted phospholipase A2 sPLA2 has been shown previously to inhibit neuron death and aspects of the inflammatory response following systemic treatment of rats with cerebral cortex lesions. In this study the properties of CHEC-9 inhibition of sPLA2 enzymes were investigated using a venom-derived sPLA2 group I and the plasma of rats and humans as the sources of enzyme activity. The results highlight the advantages of inhibitors with uncompetitive properties for inflammatory disorders including those resulting in degeneration of neurons. Methods Samples of enzyme and plasma were reacted with 1-Palmitoyl-2-Pyrenedecanoyl Phosphatidylcholine a sPLA2 substrate that forms phospholipid vesicles in aqueous solutions. Some of the plasma samples were collected from restrained peptide-treated rats in order to confirm the validity of the in vitro assays for extrapolation to in vivo effects of .