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Handbook of Microbiological Media, Fourth Edition part 153

Handbook of Microbiological Media, Fourth Edition part 153 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Rogosa Broth Modified 1515 NaCl.3.0g KCl.2.0g Yeast extract.2.0g MgSO4.0.2g Iron III chloride.0.1g Bromthymol Blue.0.04g Ampicillin solution.10.0mL Ethanol.10.0mL pH 8.0 0.2 at 25 C Source This medium without ampicillin solution and ethanol is available as a premixed powder from HiMedia. Ampicillin Solution Composition per 10.0mL Ampicillin.0.02g Preparation of Ampicillin Solution Add ampicillin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except ampicillin solution and ethanol to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 10.0mL of sterile ampicillin solution and 10.0mL of ethanol. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation cultivation and differentiation of Aeromonas species and Plesiomonas species from water samples using the membrane filter method. For the differential and selective isolation of Aer-omonas hydrophila species from water samples RM Medium Composition per liter Glucose.20.0g Agar.15.0g Yeast extract.10.0g KH2PO4.2.0g Solution 1.250.0mL Solution 2.250.0mL Solution 3.250.0mL Solution 4.250.0mL pH 6.0 0.2 at 25 C Solution 1 Composition per 250.0mL Glucose.20.0g Preparation of Solution 1 Add glucose to distilled deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Solution 2 Composition per 250.0mL Agar.15.0g Preparation of Solution 2 Add agar to distilled deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Solution 3 Composition per 250.0mL Yeast extract.10.0g Preparation of Solution 3 Add yeast extract to distilled deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. .