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Handbook of Microbiological Media, Fourth Edition part 127

Handbook of Microbiological Media, Fourth Edition part 127 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | MVTY Medium 1255 Nicotinic acid.5.0mg Riboflavin.5.0mg Thiamine-HCl.5.0mg Calcium DL-pantothenate.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100 N2. Filter sterilize. Trace Elements Solution SL-10 Composition per liter FeCl24H2O.1.5g CoCl26H2O.190.0mg MnCl24H20.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl26H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg HCl 25 solution .10.0mL Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Prepare and dispense medium under 100 N2. Add components except Wolfe s vitamin solution sodium malate solution sodium pyruvate solution NaHCO3 solution Na2S-9H2O solution and trace elements solution SL-10 to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 7.0-7.2. Gently heat and bring to boiling. Cool while sparging with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 10.0mL of sterile Wolfe s vitamin solution 10.0mL of sterile sodium malate solution 10.0mL of sterile sodium pyruvate solution 10.0mL of sterile NaHCO3 solution 10.0mL of sterile Na2S-9H2O solution and 1.0mL of sterile trace elements solution SL-10. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles. Use For the cultivation of Desulfotomaculum orientis. MVL Medium Composition per liter Agar.15.0g Pancreatic digest of casein.10.0g Yeast extract.5.0g Beef extract.2.0g Glucose.2.0g Mineral solution 1.75.0mL Mineral solution 2.75.0mL Na2CO3 8.0 solution .50.0mL Hemin 0.07 solution .10.0mL L-Cysteine-HCl-H2O 3.0 solution .10.0mL Resazurin 0.1 solution .1.0mL pH 7.0 0.2 at 25 C Mineral Solution 1 .