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Handbook of Microbiological Media, Fourth Edition part 113

Handbook of Microbiological Media, Fourth Edition part 113 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanomicrobium mobile Medium 1115 CuSO4-5H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Trace Elements Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Mix thoroughly. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.3g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to distilled deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Store anaerobically. Trimethylamine-HCl Solution Composition per 10.0mL Trimethylamine-HCl.2.0g Preparation of Trimethylamine-HCl Solution Add trimethylamine-HCl to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. NaHCO3 Solution Composition per 10.0mL NaHCO3.2.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Should be prepared freshly. Preparation of Medium Prepare and dispense medium under an oxygen-free 100 N2 gas mixture. Add components except trimethylamine-HCl solution NaHCO3 solution and Na2S-9H2O solution to 970.0mL distilled deionized water. Mix thoroughly. Sparge for 20 min with 100 N2. Adjust pH to 8.0. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Aseptically and anaerobically add 10.0mL sterile trimethylamine-HCl solution and 10.0mL Na2S-9H2O solution. Adjust pH to 8.5 using sterile NaHCO3 solution approximately 10.0mL per liter medium. Aseptically and anaerobically distribute to sterile tubes or flasks under 100 N2. Use For the cultivation ofMethanolobus taylorii. Methanomicrobium Medium Composition per liter NaHCO3.2.0g Yeast extract.1.0g Pancreatic digest of casein.1.0g NaCl.0.6g L-Cysteine-HCl-H2O.0.5g Na2S-9H2O.0.5g K2HPO4.0.3g KH2PO4.0.3g NH4 2SO4.0.3g MgSO4-7H2O.0.13g CaCl22H2O.8.0mg .