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Handbook of Microbiological Media, Fourth Edition part 112

Handbook of Microbiological Media, Fourth Edition part 112 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Methanogenium thermophilicum Medium 1105 Vitamin solution.10.0mL L-Cysteine-HCl-H2O solution.10.0mL Na2S-9H2O solution.10.0mL pH 6.9 0.2 at 25 C Trace Elements Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-2H2O.0.5g CoSO4-7H2O.0.18g ZnSO4-7H2O.0.18g FeSO4-7H2O.0.1g CaCl2-2H2O.0.1g NiCl26H2O.0.025g KAl SO4 212H2O.0.02g CuSO4-5H2O.0.01g H3BO3.0.01g Na2MoO4-4H2O.0.01g Na2SeO3-5H2O.0.3mg Preparation of Trace Elements Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Adjust pH to 7.0 with KOH. Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2Û.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12.0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. L-Cysteine-HCbH2O Solution Composition per 10.0mL L-Cysteine-HCl-H2O.0.3g Preparation of L-Cysteine-HCbH2O Solution Add L-cyste-ine-HCl-H2O to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.3g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Prepare and dispense medium under 80 H2 20 CO2 gas atmosphere. Add components except L-cysteine-HCl-H2O solution Na2S-9H2O solution and vitamin solution to distilled deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 6.9. Sparge with 80 H2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add 10.0mL .