Đang chuẩn bị liên kết để tải về tài liệu:
Handbook of Microbiological Media, Fourth Edition part 48

Handbook of Microbiological Media, Fourth Edition part 48 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | CT Agar 465 Crystal Violet.1.0mg 2 3 5-Triphenyltetrazolium chloride solution.10.0mL pH 7.0 0.2 at 25 C Source This medium without triphenyltetrazolium chloride solution is available as a premixed powder from HiMedia. Caution Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted. 2 3 5-Triphenyltetrazolium Chloride Solution Composition per 10.0mL 2 3 5-Triphenyltetrazolium chloride.0.1g Preparation of 2 3 5-Triphenyltetrazolium Chloride Solution Add 2 3 5-triphenyltetrazolium chloride to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except 2 3 5-triphe-nyltetrazolium chloride solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 10.0mL 2 3 5-triphenyltetrazolium chloride solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the detection of Gram-negative psychrotrophic bacteria causing food spoilage. CS Vitamin B12 Agar Composition per liter Glucose.20.0g K2SO4.20.0g Agar.15.0g Sodium acetate.12.0g Vitamin assay casamino acids.10.0g Papaic digest of soybean meal.5.0g Sodium thioglycolate.1.7g K2HPO4.1.0g KH2PO4.1.0g Ribonucleic acid.1.0g Sorbitan monooleate complex.1.0g MgSO4-7H2O.0.4g DL-Tryptophan.0.2g L-Cystine.0.2g FeSO4.0.02g MgSO4-7H2O.0.02g NaCl.0.02g Adenine sulfate.0.018g Guanine-HCl.0.012g Uracil.0.01g Xanthine.0.01g Pyridoxal.4.0mg Pyridoxine.4.0mg Calcium pentothenate.2.0mg Niacin.2.0mg Riboflavin.2.0mg Thiamine-HCl.2.0mg Folic acid. 1.0mg Biotin.1.0pg Lactobacillus leichmannii suspension.1.0mL pH 6.2 0.1 at 25 C Preparation of Medium Add components except Lactobacillus leichmannii suspension to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50