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Handbook of Microbiological Media, Fourth Edition part 47

Handbook of Microbiological Media, Fourth Edition part 47 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Costein s LDS Test Medium 455 Bovine serum.100.0mL Nystatin solution.1.15mL L-Cystine 1 solution .1.0mL Egg yolk emulsion.10 eggs pH 7.4 0.2 at 25 C Solution A Composition per liter Meat extract.9.0g Proteose peptone No. 3.9.0g NaCl.2.7g Glucose.1.8g Na2HPO4-12H2O.1.8g K2TeO3 2 solution .75.0mL L-Cystine 1 solution .10.0mL Caution Potassium tellurite is toxic. Preparation of Solution A Add components to distilled deionized water and bring volume to 985.0mL. Mix thoroughly. Filter sterilize. Egg Yolk Emulsion Composition Chicken egg yolks.9 Whole chicken egg.1 Preparation of Egg Yolk Emulsion Soak eggs with 1 100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Filter sterilize. Nystatin Solution Composition per 10.0mL Nystatin. 10 000U Preparation of Nystatin Solution Add nystatin to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. L-Cystine Solution Composition per 10.0mL L-Cystine.0.1g Preparation of L-Cystine Solution Add L-cystine to distilled de-ionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium To 985.0mL of sterile solution A aseptically add the remaining components. Mix thoroughly. Aseptically distribute into sterile tubes in 2.0-3.0mL volumes. Use For the isolation and cultivation of Corynebacterium diphthe-riae. Corynebacterium Medium with Blood DSMZ Medium 240 Composition per liter Agar.15.0g Casein peptone tryptic digest.10.0g Yeast extract.5.0g Glucose.5.0g NaCl.5.0g Distilled water.1000.0mL Sheep or horse blood defibrinated.50.0mL pH 7.3 0.2 at 25 C Preparation of Medium Add components except sheep or horse blood to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile sheep or horse blood. Mix thoroughly. Pour into sterile Petri dishes or .