Đang chuẩn bị liên kết để tải về tài liệu:
Handbook of Microbiological Media, Fourth Edition part 45

Handbook of Microbiological Media, Fourth Edition part 45 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Cold Filterable Tryptone Soya Broth 435 Preparation of Oxolinic Acid Solution Add oxolinic acid to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium To 930.0mL of sterile cooled Columbia agar base add sterile colistin sulfate sterile oxolinic acid and sterile de-fibrinated horse blood. Mix thoroughly. Pour into sterile Petri dishes. Use For the isolation and cultivation of streptococci in pure culture from mixed flora in clinical specimens. Colby and Zatman Agar Composition per liter Agar noble.20.0g K2HPO4.1.2g KH2PO4.0.62g NH4 2SO4.0.5g MgSO4-7H2O.0.2g NaCl.0.1g CaCl26H2O.0.05g ZnSO4-7H2O.70.0pg H3BO3.10.0pg MnSO4-5H2O.10.0pg Na2MoO4-2H2O.10.0pg CoCl2-6H2O.5.0pg CuSO4-5H2O.5.0pg FeCl3-6H2O.1.0mg Trimethylamine solution.10.0mL pH 7.0 0.2 at 25 C Trimethylamine Solution Composition per 10.0mL Trimethylamine.1.0g Preparation of Trimethylamine Solution Add trimethylamine to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except trimethylamine solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add 10.0mL of sterile trimethylamine solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Aminobacter aminovorans Bacillus species Hyphomicrobium aestuarii Hyphomicrobium facilis Hyphomi-crobium species Hyphomicrobium variabile Hyphomicrobium zavar-zinii Methylobacterium extorquens Methylobacterium species and Methylophilus methylotrophus. Colby and Zatman Medium Composition per liter Agar.15.0g K2HPO4.1.2g KH2PO4.0.62g NH4 2SO4.0.5g MgSO4-7H2O.0.2g NaCl.0.1g CaCl22H2O.34.0mg FeCl3-H2O.1.0mg Trace elements solution.1.0mL Trimethylamine 10 solution filter sterilized.10.0mL pH 7.0 0.2 at 25 C Trace Elements Solution Composition