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Handbook of Microbiological Media, Fourth Edition part 93

Handbook of Microbiological Media, Fourth Edition part 93 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | L. mono Differential HiVeg Agar Base 915 Listeria mono Enrichment Supplement II Solution Composition per 10.0mL L-phosphatidylinositol.1.0g Preparation of Listeria mono Enrichment Supplement II Solution Add L-phosphatidylinositol to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Listeria mono Selective Supplement I Solution Composition per 10.0mL Polymyxin B sulfate.76 700U Preparation of Listeria mono Selective Supplement I Solution Add polymyxin B sulfate to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Listeria mono Selective Supplement II Solution Composition per 10.0mL Ceftazidime.20.0mg Nalidixic acid sodium salt.20.0mg Amphotericin B.10.0mg Preparation of Listeria mono Selective Supplement II Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Listeria mono enrichment supplement II Listeria mono selective supplement I and Listeria mono selective supplement II to distilled deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 10.0mL Listeria mono enrichment supplement II 10.0mL Listeria mono selective supplement I and 10.0mL Listeria mono selective supplement II. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the selective and differential isolation of Listeria monocytogenes from clinical and food specimens. L. mono Confirmatory HiVeg Agar Base Composition per liter Plant special peptone.30.0g Agar.12.0g LiCl.10.0g B.C. indicator.8.6g Yeast extract.6.0g NaCl.5.0g a-Methyl-D-mannoside.3.0g Na2HPO4 anhydrous.2.5g Listeria mono enrichment supplement II.10.0mL Listeria mono selective supplement I.10.0mL Listeria mono selective supplement II.10.0mL pH 7.2 0.2 at 25 C Source This medium without Listeria mono .