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Handbook of Microbiological Media, Fourth Edition part 81

Handbook of Microbiological Media, Fourth Edition part 81 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Halomonas desiderata Medium 795 Wolfe s Vitamin Solution Composition per liter Pyridoxine-HCl.0.01g Thiamine-HCl.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg Calcium pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Thioctic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Cyanocobalamin.0.1mg Preparation of Wolfe s Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Store at 4 C. Wolfe s Mineral Solution Composition per liter MgSO4-7H2O.3.0g Nitrilotriacetic acid.1.5g NaCl.1.0g MnSO4-H2O.0.5g FeSO4-7H2O.0.1g CoCl2-6H2O.0.1g CaCl2. 0.1g ZnSO4-7H2O.0.1g CuSO4-5H2O.0.01g AlK SO4 2-12H2O.0.01g H3BO3.0.01g Na2MoO4-2H2O.0.01g Preparation of Wolfe s Mineral Solution Add nitrilotriacetic acid to 500.0mL of distilled deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled deionized water to 1.0L. Volatile Acids Solution Composition per liter a-Methylbutyric acid.0.5mL Isobutyric acid.0.5mL Isovaleric acid.0.5mL Valeric acid.0.5mL Preparation of Volatile Acids Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Combine components except the l-cysteine-HCl-H2O trimethylamine-HCl and Na2S-9H2O solution. Mix thoroughly. Gently heat and bring to boiling. Add L-cysteine-HCl-H2O. Mix thoroughly. Cool in an ice-water bath under 80 N2 20 CO2. Add trimethylamine-HCl. Mix thoroughly. Adjust pH to 7.1. Aseptically and anaerobically distribute into tubes in 10.0mL volumes under 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Immediately prior to inoculation aseptically add 0.25mL of sterile Na2S-9H2O solution to each tube. Mix thoroughly. Use For the cultivation and maintenance of Methanohalophilus mahii. Halomicrobium katesii Medium DSMZ Medium 1164 Composition per liter NaCl.195.0g MgSO4-7H2O.49.83g Proteose peptone. 5.0g Yeast extract.5.0g KCl.5.0g Glucose.1.0g CaCl2-2H2O.0.916g NaBr.0.35g NaHCO3.0.166g .