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Laboratory Exercises in Microbiology - part 5

nước dùng trẻ 24 giờ tryptic đậu nành nền văn hóa của Alcaligenes faecalis (ATCC 8750), Escherichia coli (ATCC 25922), và Pseudomonas aeruginosa (ATCC 27853) tấm thạch đậu nành tryptic tetramethyl-p-phenylenediamine dihydrochloride (thuốc thử oxidase) Bunsen ổ ghi đĩa bạch kim hoặc nhựa | Harley-Prescott Laboratory Exercises in Microbiology Fifth Edition IV. Biochemical Activities of Bacteria 30. Proteins Amino Acids and Enzymes VII Oxidase Test The McGraw-Hill Companies 2002 EXERCISE 30 Proteins Amino Acids and Enzymes VII Oxidase Test SAFETY CONSIDERATIONS Be careful with the Bunsen burner flame. No mouth pipetting. The oxidase reagent is caustic. Avoid contact with eyes and skin. In case of contact immediately flush eyes or skin with plenty of water for at least 15 minutes. Materials per Student young 24-hour tryptic soy broth cultures of Alcaligenes faecalis ATCC 8750 Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 tryptic soy agar plates tetramethyl-p-phenylenediamine dihydrochloride oxidase reagent Bunsen burner platinum or plastic loops wax pencil Pasteur pipette with pipettor Oxidase Disks or Dry Slides Difco Oxidase Test Strips KEY Scientific Products SpotTest Oxidase Reagent Difco wooden applicator sticks Whatman No. 2 filter paper Learning Objectives Each student should be able to 1. Understand the biochemistry underlying oxidase enzymes 2. Describe the experimental procedure that enables one to distinguish between groups of bacteria based on cytochrome oxidase activity 3. Give examples of oxidase-positive and oxidasenegative bacteria 4. Perform an oxidase test Suggested Reading in Textbook 1. The Electron Transport Chain section 9.5 see also figures 9.13-9.15. 2. Rapid Methods of Identification section 36.2 see also table 36.3. Pronunciation Guide Alcaligenes faecalis al-kah-LIJ-e-neez fee-KAL-iss Escherichia coli esh-er-I-ke-a KOH-lee Pseudomonas aeruginosa soo-do-MO-nas a-ruh-jin-OH-sah Why Are the Above Bacteria Used in This Exercise This exercise gives the student experience in performing the oxidase test. The oxidase test distinguishes between groups of bacteria based on cytochrome oxidase activity. Three bacteria will be used. Alcaligenes faecalis L. fae-cium of the dregs of feces is a gram-negative aerobic rod .