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Báo cáo khoa học: " Evaluation of a competitive ELISA for antibody detection against avian influenza virus"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Evaluation of a competitive ELISA for antibody detection against avian influenza virus | J. Vet. Sci. 2009 10 4 323-329 DOI 10.4142 jvs.2009.10.4.323 JOURNAL OF Veterinary Science Evaluation of a competitive ELISA for antibody detection against avian influenza virus 1 7 2 2 2 .3 3 Dae Sub Song Youn Jeong Lee Ok Mi Jeong Yong Joo Kim Chan Hee Park Jung Eun Yoo Woo Jin 2 2 _3 1 1 4 2 Jeon Jun Hun Kwon Gun Woo Ha Bo Kyu Kang Chul Seung Lee Hye Kwon Kim Byeong Yeal Jung Jae Hong Kim4 Jin Sik Oh3 Green Cross Veterinary Products Youngin 446-569 Korea 2National Veterinary Research and Quarantine Service Anyang 430-757 Korea 3Animal Genetics Suwon 443-823 Korea 4College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Active serologic surveillance is necessary to control the spread of the avian influenza virus AIV . In this study we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3 358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens or following vaccination of the AIV H9 or H5 subtypes in chickens ducks and geese. Next we tested the sensitivity and specificity of the cELISA with sera from twelve different animal species and compared these results with those obtained by the hemagglutination-inhibition HI test the gold standard in AIV sera surveillance a second commercially-available cELISA IZS ELISA or the agar gel precipitation AGP test. Compared with the HI test the sensitivities and specificities of cELISA were 95 and 96 in chicken 86 and 88 in duck 97 and 100 in turkey 100 and 87 in goose and 91 and 97 in swine respectively. The sensitivities and specificities of the cELISA in this study were higher than those of IZS ELISA for the duck turkey goose and grey partridge sera samples. The results of AGP test against duck and turkey sera also showed significant correlation with the