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Báo cáo khoa học: "Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro | J. Vet. Set. 2007 8 2 169-174 JOURNAL OF Veterinary Science Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro Mohammad Alamgir Hossain1 4 Osa mu Yamato2 Gonhyung Kim1 Masahiro Yamasaki3 Yoshimitsu Maede3 Laboratory of Veterinary Surgery College of Veterinary Medicine Chungbuk National University Chungju 361-763 Korea 2Laboratory of Clinical Pathology Department of Veterinary Clinical Sciences Faculty of Agriculture Kagoshima University 1-2124 Kohrimoto Kagoshima 890-0065 Japan 3Laboratory of Internal Medicine Department of Veterinary Clinical Sciences Graduate School of Veterinary Medicine Hokkaido University Sapporo 060-0818 Japan 4Department of Pathology and Parasitology Chittagong Veterinary and Animal Sciences University Khulshi Chittagong 4202 Bangladesh The present study evaluated the effects of infected culture supernatant of erythrocytes fractionation of culture supernatant and serum from dogs infected with Babesia gibsoni B. gibsoni on the maturation of canine reticulocytes in vitro. The SDS-PAGE demonstrated that significantly broader bands were generated by both the infected culture supernatant of erythrocytes and the serum from dogs chronically infected with B. gibsoni. The culture supernatant of erythrocytes infected with B. gibsoni strongly suppressed the maturation of reticulocytes. Prior studies showed that chronically infected serum had inhibitory effects on both the maturation of reticulocytes and the canine pyrimidine 5 -nucleotidase subclass I and purine-specific 5 -nucleotidase activity. In addition serum free infected culture supernatant of erythrocytes had an inhibitory effect on the morphological maturation of reticulocytes. These results suggest that infected serum and culture supernatant of erythrocytes might accumulate excess proteins and or metabolites as a result of the inhibited maturation of reticulocytes and .