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Báo cáo sinh học: " Rapid, widespread transduction of the murine myocardium using self-complementary Adeno-associated virus"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Rapid, widespread transduction of the murine myocardium using self-complementary Adeno-associated virus | Genetic Vaccines and Therapy BioMed Central Short paper Open Access Rapid widespread transduction of the murine myocardium using self-complementary Adeno-associated virus Lourdes M Andino1 Thomas J Conlon2 Stacy L Porvasnik3 Sanford L Boye4 William W Hauswirth4 and Alfred S Lewin 1 Address Department of Molecular Genetics and Microbiology University of Florida Gainesville FL USA 2Powell Gene Therapy Center University of Florida Gainesville FL USA 3Department of Pediatrics University of Florida Gainesville FL USA and 4Department of Ophthalmology University of Florida Gainesville FL USA Email Lourdes M Andino - LAndino@ufl.edu Thomas J Conlon - conlon@gtc.ufl.edu Stacy L Porvasnik - porvas@ufl.edu Sanford L Boye - sboye@ufl.edu William W Hauswirth - hauswrth@eye.ufl.edu Alfred S Lewin - lewin@mgm.ufl.edu Corresponding author Published 10 December 2007 Received I October 2007 Accepted 10 December 2007 Genetic Vaccines and Therapy 2007 5 1 3 doi 10.1186 1479-0556-5- 13 r This article is available from http www.gvt-journal.cOm content 5 1 13 2007 Andino et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Adeno-associated virus AAV has shown great promise as a gene transfer vector. However the incubation time needed to attain significant levels of gene expression is often too long for some clinical applications. Self-complementary AAV scAAV enters the cell as double stranded DNA eliminating the step of second-strand synthesis proven to be the rate-limiting step for gene expression of single-stranded AAV ssAAV . The aim of this study was to compare the efficiency of these two types of AAV vectors in the murine myocardium. Four day old CD-1 mice were injected with either of the two AAV constructs both expressing GFP and