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Báo cáo hóa học: " Impact of g-chain cytokines on EBV-specific T cell cultures"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Impact of g-chain cytokines on EBV-specific T cell cultures | Merlo et al. Journal of Translational Medicine 2010 8 121 http www.translational-medicine.eom content 8 1 121 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Impact of g-chain cytokines on EBV-specific T cell cultures 1 1 s -I- .2 1.3 4 13 Anna Merlo Riccardo Turrini Cristina Trento Paola Zanovello Riccardo Dolcetti Antonio Rosato Abstract Background Recent preclinical adoptive immunotherapy studies in murine models prompt to employ proper rather than as many as possible antigen-specific T cells to gain better therapeutic results. Ideally proper T cells are poorly differentiated in vitro but retain the capacity to fully differentiate into effector cells in vivo where they can undergo long-term survival and strong proliferation. Such requirements can be achieved by modifying culture conditions namely using less differentiating cytokines than IL-2. Methods To evaluate this issue in human T cell cultures we exploited a well characterized and clinical-grade protocol finalized at generating EBV-specific CTL for adoptive immunotherapy. In particular we studied the impact of IL-7 IL-15 and IL-21 compared to IL-2 on different aspects of T cell functionality namely growth kinetics differentiation activation marker expression cytokine production and short-term and long-term cytotoxicity. Results Results disclosed that the culture modifications we introduced in the standard protocol did not improve activity nor induce substantial changes in differentiation marker expression of EBV-specific CTL. Conclusions Our data indicated that the addition of g-chain cytokines other than IL-2 for the generation of EBV-specific T cell cultures did not produce the improvements expected on the basis of recent published literature. This fact was likely due to the intrinsic differences between murine and human models and highlights the need to design ad hoc protocols rather than simply modify the cytokines added in culture. Background Infusion of antigen-specific T cells proved to be .