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báo cáo khoa học: " Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen"
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen | BMC Plant Biology BioMed Central Research article Open Access Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen AbdelMounim Hamman-Khalifa Antonio Jesús Castro José Carlos Jimenez-Lopez María Isabel Rodríguez-García and Juan de Dios Alché Address Department of Biochemistry Cell and Molecular Biology of Plants Estación Experimental del Zaidín Consejo Superior de Investigaciones Científicas CSIC Profesor Albareda 1 18008 Granada Spain Email AbdelMounim Hamman-Khalifa - abdelmounin@ugr.es Antonio Jesús Castro - antoniojesus.castro@eez.csic.es José Carlos Jimenez-López - josecarlos.jimenez@eez.csic.es María Isabel Rodríguez-García - mariaisabel.rodriguez@eez.csic.es Juan de Dios Alché - juandedios.alche@eez.csic.es Corresponding author Published 25 January 2008 Received 18 July 2007 BMC Plant Biology 2008 8 10 doi 10.1186 1471-2229-8-10 Accepted 25 January 2008 This article is available from http www.biomedcentral.cOm 1471-2229 8 10 2008 Hamman-Khalifa et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Pollens from different olive Olea europaea L. cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is in addition characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars. Results RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences many of which resulted in amino .