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Báo cáo khoa học: "Analysis of adenoviral attachment to human platelets Nilly Shimony1, Gregory Elkin1, Dror Kolodkin-Gal2, Lina "

Analysis of adenoviral attachment to human platelets Nilly Shimony1, Gregory Elkin1, Dror Kolodkin-Gal2, Lina | Virology Journal BioMed Central Open Access Analysis of adenoviral attachment to human platelets Nilly Shimony1 Gregory Elkin1 Dror Kolodkin-Gal2 Lina Krasny1 Simcha Urieli-Shoval3 and Yosef S Haviv 1 Address Department of Medicine Hadassah-Hebrew University Medical Center Jerusalem Israel 91120 2Department of Virology Hadassah-Hebrew University Medical Center Jerusalem Israel 91120 and 3Department of Hematology Mount Scopus Hadassah-Hebrew University Medical Center Jerusalem Israel 91120 Email Nilly Shimony - nillys@pob.huji.ac.il Gregory Elkin - elkin@hadassah.org.il Dror Kolodkin-Gal - drorgal@pob.huji.ac.il Lina Krasny-lalushka82@yahoo.com Simcha Urieli-Shoval - simcha@hadassah.org.il Yosef S Haviv - yhaviv@hadassah.org.il Corresponding author Published 17 February 2009 Received 12 January 2009 Accepted 17 February 2009 Virology journal 2009 6 25 doi l0.ll86 l743-422X-6-25 This article is available from http www.virologyj.cOm content 6 1 25 2009 Shimony et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Systemic adenoviral Ad vector administration is associated with thrombocytopenia. Recently Ad interaction with mouse platelets emerged as a key player determining liver uptake and platelet clearance. However whether Ad can activate platelets is controversial. Thus in vitro analysis of Ad attachment to platelets is of interest. Methods We developed a direct flow cytometry assay to specifically detect Ad particles adherent to human platelets. The method was pre-validated in nucleated cells. Blocking assays were employed to specifically inhibit Ad attachment to platelets. Platelet activation was analyzed using annexin v