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Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv "

We established cell suspension culture on Panax vietnamensis and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. The biomass production and ginsenoside yield were obtained 9.8 g/L DW and 6.81 mg/g DW, respectively. The effect of initial sucrose concentrations were also investigated in suspension cultures of P. vietnamensis for biomass and production of ginseng saponin (secondary metabolite). The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial. | VNU Journal of Science Natural Sciences and Technology 23 2007 269-274 Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv. Nguyen Trung Thanh1 Nguyen Van Ket2 Paek Kee Yoeup3 Department of Biology College of Science VNU 334 Nguyen Trai Hanoi Vietnam Department of Agroforestry Dalat University 3Department of Horticulture Chungbuk National University Cheongju South Korea Received 15 August 2007 Abstract. We established cell suspension culture on Panax vietnamensis and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. The biomass production and ginsenoside yield were obtained 9.8 g L DW and 6.81 mg g DW respectively. The effect of initial sucrose concentrations were also investigated in suspension cultures of P. vietnamensis for biomass and production of ginseng saponin secondary metabolite . The final dry cell weight was increased from 5.4 to 10.3 g L with an increase of initial sucrose concentration from 20 to 50 g L but an even higher sucrose concentration of 60 g L seemed to repress the cell growth further increase of sucrose concentration upto 70 g L led to a decrease in ginsenoside accumulation and biomass production. The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM. Keywords MS strength sucrose nitrogen auxin and cytokinin. 1. Introduction Vietnamese ginseng was found at highland of Central Vietnam in 1973 and was regarded as a new species as Panax vietnamensis Ha et Grushv. 1985 . This is the most southern distribution of Panax genus Araliaceae . It is a secret medicine of the Sedang ethnic group as a miraculous life-saving plant drug used for the Tác giả liên hệ. ĐT 84-4-8582178. E-mail thanhntsh@gmail.com treatment of