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Đánh giá độc tính của thuốc trừ sâu o,p`- DDT đến sự phát triển của phôi cá sóc Oryzias curvinotus

Cá sóc Oryzias curvinotus thu nhận từ Trung tâm Công nghệ Sinh học Thành phố Hồ Chí Minh, Việt Nam, được nuôi trưởng thành và có khả năng sinh sản, cho cá đực và cái thụ tinh để tiến hành thu phôi. Thu phôi cá sóc một ngày tuổi và cho phơi nhiễm với các nồng độ o,p`- DDT là 0,01; 0,03; 0,05; 0,07; 0,1; 0,15; 0,17 µg/L và 0 µg/L (mẫu đối chứng không bổ sung o,p`- DDT). | TAP CHI SINH HOC 2019 41 2se1 amp 2se2 337 344 DOI 10.15625 0866-7160 v41n2se1 amp 2se2.14181 THE IMPACT OF o p - DDT PESTICIDE TOXICITY ON THE DEVELOPMENT OF FISH EMBRYO Oryzias curvinotus Tran Thi Thu Huong1 Nguyen Xuan Tong2 3 Nguyen Thanh Binh2 Le Hung Anh2 Dang Thi Bich Hong2 1 Department of Environment Hanoi University of Mining and Geology Ha Noi Viet Nam 2 Institute of Environmental Science Engineering and Management Industrial University of Ho Chi Minh city Ho Chi Minh city Vietnam 3 Graduate University of Science and Technology VAST Vietnam Received 10 August 2019 accepted 24 September 2019 ABTRACT The fish Oryzias curvinotus was obtained from the Biotechnology Center of Ho Chi Minh City Vietnam raised and reproduced the male fish and the female fish were fertilized to collected the embryos. The one-day-old fish embryos is harvested and exposured to differenct concentrations of o p -DDT as follows 0.01 0.03 0.05 0.07 0.1 0.15 0.17 µg L and 0 µg L the control sample . After 24 hours and 48 hours of testing most of the fish embryos were not able to survive in which the concentration of 0.17 µg L recorded the highest mortality rate of 62.2 and 75 comparing with the control sample has the survival rate of 100 . The LC 50 values at 24 hours and 48 hours were 0.0813 µg l and 0.0406 µg l respectively and the testing concentrations all strongly effected the heart rate of fish embryos. The observation of morphology and structure also found defects malformation in the spine and eyes of fish embryos. The presence of o p -DDT in water has caused disadvantages for the growth and development of aquatic animals in particular and the water ecosystem in general. Keywords Oryzias curvinotus toxicity DDT POPs pesticide. Citation Tran Thi Thu Huong Nguyen Xuan Tong Nguyen Thanh Binh Le Hung Anh Dang Thi Bich Hong 2019. The impact of o p - DDT pesticide toxicity on the development of fish embryo Oryzias curvinotus. Tap chi Sinh hoc 41 2se1 amp 2se2 337 344. https doi.org .