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Molecular characterization of isolated lactic acid bacteria from different traditional dairy products of tribes in the fars province, Iran
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The present researchaimed to investigate the morphological, biochemical and genotypic characterization of dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional yogurt, doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria, biochemical and genotypic identification were conducted. Totally, 157 LAB isolates were selected. | MOLECULAR CHARACTERIZATION OF ISOLATED LACTIC ACID BACTERIA FROM DIFFERENT TRADITIONAL DAIRY PRODUCTS OF TRIBES IN THE FARS PROVINCE IRAN Maryam Jafari1 Nabi Shariatifar1 Gholamreza Jahed Khaniki 1 Mehran sayadi 2 Abbas Abdollahi3 Address es Gholamreza Jahed Khaniki Mehran Sayadi 1 Department of Food Safety and Hygiene School of Public Health Tehran University of Medical Sciences Tehran Iran. 2 Department of Food Safety and Hygiene Faculty of Health Fasa University of Medical Sciences Fasa Iran 3 Department of Microbiology School of Medicine Fasa University of Medical Sciences Fasa Iran. Corresponding author Ghjahedkh@yahoo.com Mehransayadi6262@yahoo.com https doi.org 10.15414 jmbfs.3621 ARTICLE INFO ABSTRACT Received 22. 8. 2020 Various traditional dairy products could be considered as an abundant source for Isolation collection of new lactic acid bacteria LAB Revised 3. 5. 2021 with unique characteristics. The present researchaimed to investigate the morphological biochemical and genotypic characterization of Accepted 11. 5. 2021 dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional Published 1. 10. 2021 yogurt doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria biochemical and genotypic identification were conducted. Totally 157 LAB isolates were selected. Cocci and rod shape LAB were 53.50 and 46.49 respectively. Biochemical tests showed the occurrence of 40.6 of the strains were Streptococcus 2.25 of the strains were enterococci Regular article and 20.3 of the strains were lactococci. Most isolated lactobacilli were related to Lactobacillus plantarum and Lactobacillus bulgaricus. Genetically the presence of the following species was verified Lactococcus lactis subsp. cremoris Lactobacillus plantarum Streptococcus thermophilus Lactobacillus fermentum Lactococcus lactis subsp. lactis Lactobacillus bulgaricus Lactobacillus helveticus