Đang chuẩn bị liên kết để tải về tài liệu:
Extracellular glutaminase-free l-asparaginase from trichoderma viride F2: Purification, biochemical characterization and evaluation of its potential in mitigating acrylamide formation in starchy fried food

The purified L-asparaginase is monomeric with a molecular mass of 57 kDa and exhibited optimum activity at pH 7.5 and 37 °C, which is relatively close to the human body's internal environment. The purified Lasparaginase showed high affinity and catalytic efficiency towards its natural substrate L-asparagine with Km and Vmax of 1.2 mM and 71.3 U/mL, respectively, and did not exhibit any intrinsic glutaminase activity |