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Transcriptome profiling of Stevia rebaudiana MS007 revealed genes involved in flower development

Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence, this study aims to identify the genes involved in the flowering of local S. rebaudiana accession MS007 by investigating the transcriptomic data of two stages of growth, before flowering (BF) and after flowering (AF) that were deposited under accession number SRX6362785 and SRX6362784 at the NCBI SRA database. | Turkish Journal of Biology Turk J Biol 2021 45 314-322 http journals.tubitak.gov.tr biology TÜBİTAK Research Article doi 10.3906 biy-2103-3 Transcriptome profiling of Stevia rebaudiana MS007 revealed genes involved in flower development 1 1 2 Nurul Hidayah SAMSULRIZAL Khairul Shahyidi KHADZRAN 1 1 3 Tamil Chelvan MEENAKSHI SUNDRAM Zarina ZAINUDDIN Siti Hajar Nor SHAARANI 1 2 Nur Sabrina Ahmad AZMI Sarahani HARUN 1 Department of Plant Science Kulliyyah of Science International Islamic University Malaysia Kuantan Pahang Malaysia 2 Centre for Bioinformatics Research Institute of Systems Biology National University of Malaysia Bangi Selangor Malaysia 3 Faculty of Chemical and Process Engineering Technology Malaysia University Pahang Malaysia Received 02.03.2021 Accepted Published Online 28.04.2021 Final Version 23.06.2021 Abstract Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence this study aims to identify the genes involved in the flowering of local S. rebaudiana accession MS007 by investigating the transcriptomic data of two stages of growth before flowering BF and after flowering AF that were deposited under accession number SRX6362785 and SRX6362784 at the NCBI SRA database. The transcriptomic study managed to annotate 108299 unigenes of S. rebaudiana with 8871 and 9832 genes that were differentially expressed in BF and AF samples respectively. These genes involved in various metabolic pathways related to flower development response to stimulus as well as photosynthesis. Pheophorbide A oxygenase PAO eukaryotic translation initiation factor 3 subunit E TIF3E1 and jasmonate ZIM domain-containing protein 1 JAZ1 were found to be involved in the flower development. The outcome of this study will help