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IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice

The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferationassociated signaling pathway components JNK1, NF-κB and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the IL-1R1 KO mice at different time points (p | Turkish Journal of Biology Turk J Biol 2021 45 225-234 http journals.tubitak.gov.tr biology TÜBİTAK Research Article doi 10.3906 biy-2010-51 IL-1R1 deficiency impairs liver regeneration after 2 3 partial hepatectomy in aged mice 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 Deming LI Ze WANG Chunyan ZHANG Cunshuan XU 1 State Key Laboratory Cell Differentiation and Regulation Henan Normal University Xinxiang Henan China 2 Henan International Joint Laboratory of Pulmonary Fibrosis Henan Normal University Xinxiang Henan China 3 Henan Center for Outstanding Overseas Scientists of Pulmonary Fibrosis Henan Normal University Xinxiang Henan China 4 College of Life Science Henan Normal University Xinxiang Henan China 5 Institute of Biomedical Science Henan Normal University Xinxiang Henan China 6 Overseas Expertise Introduction Center for Discipline Innovation of Pulmonary Fibrosis 111 Project Henan Normal University Xinxiang Henan China Received 22.10.2020 Accepted Published Online 03.02.2021 Final Version 20.04.2021 Abstract Inflammation has a dual effect it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration LR after partial hepatectomy PH in aged mice. The wild-type WT n 36 and the IL-1R1 knockout KO n 36 24-month-old C57BL 6J mice underwent two-thirds PH 33 WT mice underwent sham operation. Liver coefficient was calculated by liver body weight. The mRNA and protein expressions of genes were evaluated by quantitative real- time polymerase chain reaction qRT-PCR and Western blotting methods respectively. Compared with WT mice liver coefficient was lower in the IL-1R1 KO aged mice at 168 and 192 h p 0.039 and p 0.027 . The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferation- associated signaling pathway components JNK1 NF-κB and STAT3 reduced or .