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Multiple imputation and direct estimation for qPCR data with non‑detects

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Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. An important aspect of qPCR data that has been largely ignored is the presence of non-detects: Reactions failing to exceed the quantifcation threshold and therefore lacking a measurement of expression. |

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Multiple imputation and direct estimation for qPCR data with non‑detects

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