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Standardization of Cytokinins (BAP and Kinetin) concentrations and their combination with NAA on regeneration through seeds in lime (Citrus aurantifolia)

In vitro regeneration through seeds were studied in lime (Citrus aurantifolia) by culturing lime seeds on MS media supplemented with BAP (1, 3 and 5 mg/l), Kinetin (1, 3 and 5 mg/l) and their combination with NAA (0.1, 0.5 and 1 mg/l). In reference to increased germination percentage, mean shoot length, mean number of leaves per explant and mean root length, among BAP and Kinetin concentrations, BAP 3 mg/l was found to be the best treatment. In terms of cytokinins and NAA combine effect, BAP 5 mg/l + NAA 0.5 mg/l and Kinetin 3 mg/l + NAA at 0.1 mg/l were found to be the best combinations. Survival was 100 per cent when seeds were used as explant material. |