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Influence of storage duration and storage temperature on in-vitro pollen germination of citrus species
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The aim of present study was to access the pollen in- vitro germination of pollen grains of five citrus species belonging to the family Rutaceae viz., mandarin (citrus. reticulata Blanco) sweet orange (C. sinensis L. Osbeck) grapefruit (C. paradisi Macf.) pummelo (C. maxima Burm. Merr.) and tangelo (C. reticulata × C. maxima or × C. paradise) under low temperature storage conditions during year 2014. Pollen germination were checked up to 48 weeks, stored at different temperatures like 4ºC, -20ºC, and -196ºC. Pollen stored at low temperature (-196℃ and -20℃) showed better germination percentage as compared to pollen stored at 40C and room temperature. |