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Stimulation of shoot regeneration through leaf thin cell layer culture of Passiflora edulis sims

In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture. Moreover, the light (fluorescent lamps with photoperiod of 16 hours/day and lighting intensity of 40 - 45 µmol.m-2 .s-1 ) improved not only morphogenesis rate, but also shoot regeneration rate (100%) of leaf explants after 8 weeks of culture. This study provided a novel method for rapid micro-propagation of P. edulis Sims. | Stimulation of shoot regeneration through leaf thin cell layer culture of Passiflora edulis sims