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Optimization for production and partial purification of laccase from ash gourd peels

The aim of the present study was to optimize a process for production enzyme Laccase via microbial fermentation using peels of Ash Gourd. It is rich in various nutritional substances which are required for culture of microorganisms By estimating proximate values of various substances a synthetic media was prepared for Trichoderma Viride. Laccases were collected extra and intracellular level. The enzyme activity, growth was observed which were treated in various conditions and purified via various steps i.e. Ammonium Sulphate and dialysis. After dialysis activity in different conditions were 0.1N HCl- 30.17, 0.1N NaOH-23.17, Boiled22.05, Experimental Noramal- 22.05, and Sonicated- 37.09. | Optimization for production and partial purification of laccase from ash gourd peels