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Growth of Trichoderma spp on different solid substrates
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Growth of Trichoderma spp on different solid substrates
Thanh Nga
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Trichoderma is one of the common fungal biocontrol agents being used worldwide for efficient management of various foliar and soil borne plant pathogens. Unlike chemicals, the establishment of these biocontrol agents in the targeted niche requires support even after their application. Two Trichoderma species (Isolate 2 and Isolate 5) were isolated from paddy and groundnut rhizospheres respectively. These two isolates were the two most vigorous ones among all the seven Trichoderma isolates obtained from different crop rhizospheres on the basis of their growth in different media and their inhibitory effects on two potential soil borne pathogens namely Rhizoctonia solani and Sclerotium rolfsii as observed in dual culture experiment. | Int.J.Curr.Microbiol.App.Sci 2019 8 9 2519-2529 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 09 2019 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2019.809.292 Growth of Trichoderma spp on Different Solid Substrates B. Boblina S. K. Beura M. K. Mishra and A. G. Panda Department of Plant Pathology College of Agriculture Orissa University of Agriculture and Technology Bhubaneswar Odisha-751003 India Corresponding author ABSTRACT Keywords Rhizoctonia solani ecosystem disease management Article Info Accepted 24 AUgust 2019 Available Online 10September 2019 Trichoderma is one of the common fungal biocontrol agents being used worldwide for efficient management of various foliar and soil borne plant pathogens. Unlike chemicals the establishment of these biocontrol agents in the targeted niche requires support even after their application. Two Trichoderma species Isolate 2 and Isolate 5 were isolated from paddy and groundnut rhizospheres respectively. These two isolates were the two most vigorous ones among all the seven Trichoderma isolates obtained from different crop rhizospheres on the basis of their growth in different media and their inhibitory effects on two potential soil borne pathogens namely Rhizoctonia solani and Sclerotium rolfsii as observed in dual culture experiment. Present study deals with evaluation of eighteen solid substrates. Colony forming units were counted following the serial dilution technique and a gradual decrease was observed over a period of six months. Among different solid substrates tested vermicompost was observed to be the best solid substrate for isolate 2 recording the maximum cfu count 463.67 x 107cfu g of substrate in the first month after inoculation and it retained spore viability even after six months of inoculation giving a fairly good cfu count of 97.33 x107cfu g. For isolate 5 groundnut shell performed the .
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