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A green and simple synthesis of chitosan/Ag nanocomposites and study for their antibacterial activity on staphylococcus aureus and escherichia coli

A green and simple approach has been successfully developed to synthesize chitosan/Ag nanocomposites using kumquat extract as a biological reducing agent. It indicates to be an ecofriendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size ~15-25 nm. | A green and simple synthesis of chitosan/Ag nanocomposites and study for their antibacterial activity on staphylococcus aureus and escherichia coli Vietnam Journal of Science and Technology 56 (2A) (2018) 89-98 A GREEN AND SIMPLE SYNTHESIS OF CHITOSAN/Ag NANOCOMPOSITES AND STUDY FOR THEIR ANTIBACTERIAL ACTIVITY ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI Tran Thi Bich Quyen1, *, Tran Quang Thanh1, Ha Thanh Toan2, Doan Van Hong Thien1, Nguyen Trong Tuan3 1 Department of Chemical Engineering, College of Technology, Can Tho University, 3/2 Street, Ninh Kieu District, Can Tho City, Viet Nam 2 Biotechnology Research and Development Institute, Can Tho University, 3/2 Street, Ninh Kieu District, Can Tho City, Viet Nam 3 Department of Chemistry, College of Natural Sciences, Can Tho University, 3/2 Street, Ninh Kieu District, Can Tho City, Viet Nam * Email: ttbquyen@ctu.edu.vn Received: 4 April 2018; Accepted for publication: 12 May 2018 ABSTRACT A green and simple approach has been successfully developed to synthesize chitosan/Ag nanocomposites using kumquat extract as a biological reducing agent. It indicates to be an eco- friendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size ~15-25 nm. Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against S. aureus and E. coli. The chitosan/Ag nanocomposite was found to have significantly higher antimicrobial activity than its components at their respective concentrations. The presence of a small percentage (2.5 %, w/w) of metal nanoparticles in the nanocomposite was enough to significantly enhance inactivation of S. aureus and E. coli as .