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Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer

Using quantitative methylation-specific PCR (QM-MSP) is a promising method for colorectal cancer (CRC) diagnosis from stool samples. Difficulty in eliminating PCR inhibitors of this body fluid has been extensively reported. | Improved amplification efficiency on stool samples by addition of spermidine and its use for non-invasive detection of colorectal cancer