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Mass multiplication of entomopathogenic nematodes on in vitro solid media

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In this study, two Haryana local isolates of entomopathogenic nematodes (EPNs) namely Steinernema abbasi and Heterorhabdus indica were mass multiplied on eight culture media compromising of Wout’s medium, Modified Wout’s medium I, Modified Wout’s medium II, Modified Wout’s medium III, Egg yolk medium I, Egg yolk medium II, Modified egg yolk medium and Modified dog biscuit medium and harvested at two different time intervals i.e. at 30 days and 60 days interval. | Mass multiplication of entomopathogenic nematodes on in vitro solid media