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Study of genetic polymorphism in wheat and its wild relatives using ISSR markers

Inter Simple Sequence Repeats, the PCR based technique was used to reveal the genetic diversity among wheat and its wild relatives. DNA profiling of 27 wheat genotypes at all the existing ploidy levels (diploid, tetraploid and hexaploid) was done using ISSR primers. All the primers were polymorphic. The primers resulted in the amplification of 73 bands out of which 17 bands were unique. Unique bands resulted in case of all the primers. According to these 4 ISSR primers genetic similarity value was observed ranging from 0 to 1, the highest genetic similarity value (1.00) was noticed between T. timopheevii and T. sphaerococcum followed by 0.909 between T. tauschii - T. timopheevii and T. tauschii -T. sphaerococcum. The lowest genetic similarity value (0.00) was noticed between PBN 51 and NIAW 34 owing to their different places of release. It was followed by 0.032 between PBW 373 and PDW 291 as one is hexaploid and the other one is tetraploid wheat. | Study of genetic polymorphism in wheat and its wild relatives using ISSR markers