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Characterization of Rhizosphere soils of foxtail millet and isolation of Azospirillum strains

A Total of 40 different foxtail millet roots samples along with rhizospheric soil were collected from Raichur and Koppal districts of Karnataka. Azospirillum strains were isolated and physic-chemical properties of the soil were analyzed. Among them, 20 samples each from Raichur and Koppal districts were collected. The physico-chemical properties of the soil and available nutrients of Raichur district samples ranged from, pH (7.58-8.85), electrical conductivity (0.12-0.99 dS m-1 ), organic carbon (0.11-0.87%), available nitrogen (110.40 to 358.80 kg ha-1 ), available phosphorus content (9.35 to 29.92 kg ha-1 ), available potassium (117.60 to 362.88 kg ha-1 ). Likely, the physico-chemical properties of the soil and available nutrients of Koppal district samples ranged from, pH (6.30-8.50), electrical conductivity (0.12-0.85 dS m-1 ), organic carbon (0.12-0.91%), available nitrogen (188.60 to 552.10 kg ha-1 ), available phosphorus content (8.66 to 35.07 kg ha-1 ), and available potassium (117.60 to 406.54 kg ha-1 ). Forty Azospirillum isolates were obtained by adopting enrichment culture technique. All the isolates formed subsurface pellicles in NFBTB medium including the reference strain. Pellicle was formed 1-2 mm from the surface of semisolid NFBTB medium. All the Azospirillum strains turned olive green colour of Bomothymol Blue (BTB) to brilliant blue. | Characterization of Rhizosphere soils of foxtail millet and isolation of Azospirillum strains