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Molecular characterization and dna fingerprinting of superior jackfruit genotypes from Kerala using SSR markers

The PCR products obtained from SSR analysis were separated on 3 percent high resolution agarose gel and the amplification patterns were observed. Eleven SSR primers which showed maximum polymorphism were selected for fingerprinting. The amplification pattern obtained with these primers were scored and depicted to develop fingerprint for each variety. Most of the amplicons were found to be shared among the genotypes. However, the pattern of sharing was different and good enough to separate out most of the varieties. Unique amplicons were observed for Sindhur, Pathamuttom varikka1, Pathamuttom varikka-2 and Chengalam varikka, which can act as specific fingerprint of these genotypes. Among the SSR primers, MAA145 showed 100% polymorphism. The PIC value for SSR primers ranged from 0.22 to 0.98 with an average of 0.79 and MI value varied from 0.66 to 2.7 with an average of 1.18. | Molecular characterization and dna fingerprinting of superior jackfruit genotypes from Kerala using SSR markers