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Assessment of compatibility of Trichoderma species with different fungicides in vitro

The test organism Trichoderma was isolated from the soil using Trichoderma selective medium, observations for radial growth, colony characters and pigmentation were recorded on potato dextrose agar medium. For assessing the compatibility of Trichoderma species, eight fungicides (carbendazim, carboxin, triadimefon, thiophanate methyl, chlorothalonil, copper- oxychloride, mancozeb, and wettable sulphur) were used each at three concentrations 500, 1000 and 1500 ppm, respectively in addition to control and poisoned food techniques was followed in vitro. All the fungicides significantly reduce the radial growth of T. viride and T. harzianum except wettable sulphur and mancozeb. There was no radial growth of T. viride and T. harzianum in carbendazim, carboxin and thiophanate methyl, while maximum mean radial growth of T. viride (85.22 mm) and T. harzianum (79.77 mm) were recorded in wettable sulphur and mancozeb. All the three concentrations significantly reduced the radial growth of 41.15 mm in T. viride and 34.66 mm in T. harzianum being maximum in 1500 ppm. The significant interaction component clearly revealed that with increasing concentration of each fungicide, there was a significant reduction in radial growth. | Assessment of compatibility of Trichoderma species with different fungicides in vitro