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Evaluation of genetic diversity in some banana hybrids using ISSR markers

Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Inter simple sequence repeat (ISSR) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 10 primers were evaluated for banana hybrids based on ISSR polymorphism. Diversity analysis carried out by using ISSR markers in some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 10 primers studied, six primers exhibited scorable markers. A total of 54 markers were produced. The markers ranged from 5 to 8 in different primers. Of the 54 markers produced, 30 were polymorphic which account to 53.83 per cent polymorphism. Among the six primers studied ISSR 812 (UBC- 812) produced maximum number of polymorphic bands among the banana genotypes and might be useful for genetic diversity or DNA finger printing studies. | Evaluation of genetic diversity in some banana hybrids using ISSR markers