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Optimization of an efficient rapid regeneration of Indian wheat cultivars by callus induction and multiple shoot induction using mature embryos
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Wheat is the second most produced staple food crop in world. An efficient method for in vitro micro propagation and genetic transformation of wheat are crucial for both basic and applied research. The present study was initiated with a view to develop a regeneration system in 4 genotypes of wheat cultivars HD2888, HD2329, DBW148, and DBW88 as a prerequisite to transformation. The mature embryos were excised from seeds and cultured on MS medium supplemented with high and low concentrations of cytokinins and auxins respectively. | Optimization of an efficient rapid regeneration of Indian wheat cultivars by callus induction and multiple shoot induction using mature embryos