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Evaluation of antibiotic resistance of gram – negative bacteria isolated from Dong Nai river

This study assessed the current status of antibiotic resistance of Gram-negative bacteria on Dong Nai–Saigon river. Water samples were collected from 4 different areas on the river including: Nam Cat Tien forest, La Nga district (Dong Nai province), Sai Gon river (HCMC) and Can Gio district (Ho Chi Minh city). | 78 EVALUATION OF ANTIBIOTIC RESISTANCE OF GRAM – NEGATIVE BACTERIA ISOLATED FROM DONG NAI RIVER Vo Thi Thu Thao, Pham Thi Hoa International University - Vietnam National University, Ho Chi Minh city Email: pthoa@hcmiu.edu.com ABSTRACT This study assessed the current status of antibiotic resistance of Gram-negative bacteria on Dong Nai–Saigon river. Water samples were collected from 4 different areas on the river including: Nam Cat Tien forest, La Nga district (Dong Nai province), Sai Gon river (HCMC) and Can Gio district (Ho Chi Minh city). Sampling sites are close to large aquaculture areas, ranging from upstream to downstream of the river. The results showed that 20 strains of Gram negative bacteria, which belong to five bacterial species, were identified: Vibrio sp, Serratia sp Enterobacteriaceae sp, Pseudomonas sp and Escherichia coli. Sensitivity to antibiotics was determined with four common antibiotics used in aquaculture including Ampicillin, Amoxicillin, Chloramphenicol (CHL), Tetracycline (TET) and Kanamycin (KAN). AMP antimicrobial resistance was highest (55%), and lowest was TET (25%). Most isolated strains (80%) were resistant to at least one antibiotic, of which 3 were resistant to all antibiotics. Antimicrobial resistance rates in the five-isolated species have shown greater concern needed for the protection of the environment and human health, particularly the antibiotics used in agriculture and aquaculture activities. Keywords: Dong Nai river, Antibiotic resistance, Gram-negative bacteria, Ampicillin, Amoxicillin. TÓM TẮT Đề tài này đánh giá về hiện trạng kháng thuốc kháng sinh của các khuẩn Gram âm trên sông Đồng Nai – Sài Gòn. Mẫu nước được thu thập từ 4 khu vực khác nhau trên sông Đồng Nai bao gồm: Rừng Nam Cát Tiên, huyện La Ngà (tỉnh Đồng Nai), sông Sài Gòn (Tp. HCM) và huyện Cần Giờ (Tp. Hồ Chí Minh). Các vị trí lấy mẫu gần với các khu vực nuôi trồng thủy sản lớn, kéo dài từ thượng nguồn cho tới hạ nguồn của sông. Kết quả phân lập được 20 .