Đang chuẩn bị liên kết để tải về tài liệu:
The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum

This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. | M. YILDIZ, Ç. SAĞLIK, C. TELCİ, E. G. ERKILIÇ Research Article Turk J Bot 35 (2011) 211-218 © TÜBİTAK doi:10.3906/bot-1005-26 The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum Mustafa YILDIZ1,*, Çağlayan SAĞLIK2, Cansu TELCİ1, Emine Gülden ERKILIÇ2 1 University of Ankara, Faculty of Agriculture, Department of Field Crops, 06110 Dışkapı, Ankara - TURKEY 2 University of Ankara, Graduate School of Biotechnology, Department of Basic Biotechnology, 06500 Beşevler, Ankara - TURKEY Received: 18.05.2010 Accepted: 08.11.2010 Abstract: This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explants. Competition among explants was achieved by varying the spacing among the explants cultured. Four different culture spacing distances were used: 0.5, 1.0, 1.5, and 2.0 cm. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length, total shoot number per petri dish, and total chlorophyll content were recorded. The results showed that encouraging competition among explants by decreasing spacing among them from 2.0 cm to 1.0 cm increased shoot number per hypocotyl, regenerated shoot length, and total shoot number per petri dish in both cultivars. When explants were cultured at 0.5 cm spacing, significant stress-initiated decreases were observed in all parameters examined. This study showed that the success of tissue culture studies for related genotype could be increased not only by determination of correct concentrations and combinations of auxins and cytokinins in growth medium but also by evaluating competition among explants cultured. Key words: Linum usitatissimum, hypocotyl, in vitro competition, shoot regeneration In vitro rekabetin Linum usitatissimum hipokotil eksplantlarından sürgün .