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RAPD and ISSR analysis of some economically important species, varieties, and cultivars of the genus Allium (Alliaceae)

RAPD and ISSR markers have been used to reveal the genetic diversity and phylogenetic analysis of some economically important species of Allium. Two plants related to Allium were also included for better understanding of the phylogeny. | Turkish Journal of Botany Turk J Bot (2013) 37: 605-618 © TÜBİTAK doi:10.3906/bot-1208-18 http://journals.tubitak.gov.tr/botany/ Research Article RAPD and ISSR analysis of some economically important species, varieties, and cultivars of the genus Allium (Alliaceae) 1,3 2 3 3 Ashutosh MUKHERJEE , Biswanath SIKDAR , Biplab GHOSH , Anuradha BANERJEE , 3 3 3, Enakshi GHOSH , Moimee BHATTACHARYA , Satyesh Chandra ROY * 1 Department of Botany, Dinabandhu Mahavidyalaya, Bongaon, West Bengal, India 2 Department of Genetic Engineering and Biotechnology, University of Rajsahi, Bangladesh 3 Department of Botany, University of Calcutta, Kolkata, West Bengal, India Received: 09.08.2012 Accepted: 26.01.2013 Published Online: 02.07.2013 Printed: 02.08.2013 Abstract: RAPD and ISSR markers have been used to reveal the genetic diversity and phylogenetic analysis of some economically important species of Allium. Two plants related to Allium were also included for better understanding of the phylogeny. ISSR showed more polymorphism within A. cepa and A. sativum, while RAPD showed more polymorphism within A. porrum. Overall, RAPD revealed more intraspecific diversity than ISSR, while ISSR showed more interspecific diversity than RAPD. This showed the usefulness of using both markers for the study of Allium. UPGMA-based dendrograms showed a close relationship between Allium sativum and Allium porrum. A. porrum showed less genetic variability than A. cepa and A. sativum. Several unique bands were identified with RAPD and ISSR, which can be converted into cultivar-specific markers. Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. This study showed that rapid and cost-effective markers like RAPD and ISSR coupled with appropriate statistical tools can be successfully applied to study phylogenetic relationships at the interspecific and intraspecific level in Allium. These markers proved to be suitable for both phylogenetic .