Đang chuẩn bị liên kết để tải về tài liệu:
Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ

The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. | Turkish Journal of Biology Turk J Biol (2014) 38: 177-184 © TÜBİTAK doi:10.3906/biy-1305-46 http://journals.tubitak.gov.tr/biology/ Research Article Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3 1,2 1 2 1, Serpil UĞRAŞ , Kazım SEZEN , Hatice KATI , Zihni DEMİRBAĞ * Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey 2 Department of Biology, Faculty of Arts and Science, Giresun University, Giresun, Turkey 1 Received: 20.05.2013 Accepted: 30.10.2013 Published Online: 28.03.2014 Printed: 28.04.2014 Abstract: The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. A highly active antibacterial substance produced by Sm-Mm3 was purified by ammonium sulfate precipitation and high-performance liquid chromatography, respectively, and identified by spectrometric analysis (TOF-MS). Finally, we showed that this molecule is heat-stable, stable in the pH range of 5–9, and insensitive to organic solvents and proteinase K. The molecular mass was determined as 479 Da. Plasmid elimination demonstrated that the gene responsible for this antibacterial substance of the Sm-Mm3 strain is located on the plasmid. Based on the determined characteristics, the antibacterial substance was identified as a bacteriocin-like substance. Although this active substance is similar to bacteriocin because of many features such as mode of action and physicochemical properties, it differs by having a low molecular weight and by being insensitive to proteinase K. This active substance has potential to control many different pathogenic bacteria in various areas, particularly in agriculture, against plant pathogenic bacteria. Key words: Antibacterial substance, biological .

TÀI LIỆU LIÊN QUAN

Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity

Production, purification and characterization of an antibacterial compound from streptomyces griseus TBG19NRA1 isolated from the forest soils of the western ghats of Kerala, India

Optimization, purification and characterization of phytase from isolated probiotic Pediococcus acidilactici BNS5B

Purification and characterization of laccase involved in the decolourization of synthetic dyes and 2,3,7,8-TCDD congener degradation by the white rot fungus isolated from Bavi forest of Vietnam

Purification and characterization of mitochondrial thioredoxin reductase enzyme from rainbow trout (Oncorhynchus mykiss) liver and investigation of the in vitro effects of some metal ions on the enzyme

Partial purification and characterization of cellulases from digestive tracts of the African giant snail (Achatina achatina)

Partial purification and characterization of proteases from the visceral waste of Indian major carp, Labeo Rohita (Hamilton, 1822)

Expression, purification, and characterization of rhTyrRS

Expression, purification and biological characterization of the extracellular domain of CD40 from Pichia pastoris

Partial purification and characterization of two pectinases (polygalacturonase and pectin lyase) from papaya pericarp (Carica papaya cv. solo 8)