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Characterization, antifungal activity and cell immobilization of a chitinase from Serratia marcescens MO-1

Chitinases have the potential to control many pathogen species, such as fungi containing chitin on their cell walls. In the present study, chitinase from novel isolate Serratia marcescens MO-1, isolated from Poecilimon tauricola (Orthoptera: Tettigoniidae) in Turkey, was investigated. | Turkish Journal of Biology Turk J Biol (2013) 37: 639-644 © TÜBİTAK doi:10.3906/biy-1208-45 http://journals.tubitak.gov.tr/biology/ Research Article Characterization, antifungal activity, and cell immobilization of a chitinase from Serratia marcescens MO-1 1,2 1, 1 Sezer OKAY , Murat ÖZDAL *, Esabi Başaran KURBANOĞLU Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey 2 Department of Biology, Faculty of Science, Çankırı Karatekin University, Çankırı, Turkey 1 Received: 20.08.2012 Accepted: 27.01.2013 Published Online: 08.10.2013 Printed: 04.11.2013 Abstract: Chitinases have the potential to control many pathogen species, such as fungi containing chitin on their cell walls. In the present study, chitinase from novel isolate Serratia marcescens MO-1, isolated from Poecilimon tauricola (Orthoptera: Tettigoniidae) in Turkey, was investigated. The optimum pH and temperature for the chitinase activity were found to be pH 7.0 (36.6 U/mL) and 50 °C (37.1 U/mL), respectively. Moreover, the activity was still high in acidic (23.0 U/mL at pH 3.0) and basic (17.3 U/mL at pH 11.0) conditions as well as at lower (29.5 U/mL at 20 °C) and higher (28.7 U/mL at 80 °C) temperatures. The enzyme was highly thermotolerant, keeping 63.7% (23.5 U/mL) of its activity after incubation at 90 °C for 15 min. Antifungal activity of the chitinase against Alternaria citri, Fusarium oxysporum, Trichoderma harzianum, Aspergillus niger, and Rhizopus oryzae was determined. In addition, chitinase production by immobilized S. marcescens MO-1 cells was monitored over 10 days; the enzyme activity was found to be 36–41 U/mL during this period. Our results showed that the chitinase from S. marcescens MO-1 seems to be valuable in terms of its biotechnological applications; it can be produced using immobilized cells and can be used against fungal pathogens as an alternative to chemical pesticides. Key words: Serratia marcescens, chitinase, cell immobilization, .