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Biopolymer generation from sweet sorghum juice: screening, isolation, identification, and fermentative polyhydroxyalkanoate production by Bacillus aryabhattai

This study aims to use sweet sorghum juice (SSJ) to produce an environmentally friendly biopolymer of polyhydroxyalkanoates (PHAs) by bacteria isolated from soil. Among 18 isolates, only 3 isolates, coded as S4, S6, and S11, were chosen to produce PHA via batch fermentation compared to 3 strains of Alcaligenes eutrophus, A. latus, and Hydrogenophaga sp., respectively, under controlled conditions at pH 7, 35 °C, and 20 g/L of initial total sugar in the SSJ. | Turkish Journal of Biology Turk J Biol (2013) 37: 259-264 © TÜBİTAK doi:10.3906/biy-1205-62 http://journals.tubitak.gov.tr/biology/ Research Article Biopolymer generation from sweet sorghum juice: screening, isolation, identification, and fermentative polyhydroxyalkanoate production by Bacillus aryabhattai 1 2 3 4, Varavut TANAMOOL , Tsuyoshi IMAI , Paiboon DANVIRUTAI , Pakawadee KAEWKANNETRA * 1 Graduate School of Khon Kaen University, Khon Kaen 40002, Thailand 2 Division of Environmental Science and Engineering, Yamaguchi University, Tokiwadai, Ube, Yamaguchi 755-8611, Japan 3 Fermentation Research Center for Value Added Agricultural Products, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand 4 Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand Received: 29.05.2012 Accepted: 16.08.2012 Published Online: 16.05.2013 Printed: 17.06.2013 Abstract: This study aims to use sweet sorghum juice (SSJ) to produce an environmentally friendly biopolymer of polyhydroxyalkanoates (PHAs) by bacteria isolated from soil. Among 18 isolates, only 3 isolates, coded as S4, S6, and S11, were chosen to produce PHA via batch fermentation compared to 3 strains of Alcaligenes eutrophus, A. latus, and Hydrogenophaga sp., respectively, under controlled conditions at pH 7, 35 °C, and 20 g/L of initial total sugar in the SSJ. The results clearly showed that the isolate S4 can produce maximum PHAs of 1.74 g/L with 57.62% dry cell weight and yield maximum hourly productivity at 0.097 g/L. S4 was further identified by full-length 16S rDNA gene sequence homology, and the findings showed that it was closely related to Bacillus aryabhattai (99.70% similarity). Interestingly, there has been as of yet no report on the use of this strain to produce an added-value biopolymer of PHA. Key words: Biopolymer, polyhydroxyalkanoates, sweet sorghum juice, fermentation, Bacillus aryabhattai 1. Introduction Synthetic plastics, .